Subcellular Localization Screening of Colletotrichum higginsianum Effector Candidates Identifies Fungal Proteins Targeted to Plant Peroxisomes, Golgi Bodies, and Microtubules.
Identifieur interne : 000163 ( Main/Exploration ); précédent : 000162; suivant : 000164Subcellular Localization Screening of Colletotrichum higginsianum Effector Candidates Identifies Fungal Proteins Targeted to Plant Peroxisomes, Golgi Bodies, and Microtubules.
Auteurs : Guillaume P. Robin [France] ; Jochen Kleemann [Allemagne] ; Ulla Neumann [Allemagne] ; Lisa Cabre [France] ; Jean-Félix Dallery [France] ; Nicolas Lapalu [France] ; Richard J. O'Connell [France]Source :
- Frontiers in plant science [ 1664-462X ] ; 2018.
Abstract
The genome of the hemibiotrophic anthracnose fungus, Colletotrichum higginsianum, encodes a large inventory of putative secreted effector proteins that are sequentially expressed at different stages of plant infection, namely appressorium-mediated penetration, biotrophy and necrotrophy. However, the destinations to which these proteins are addressed inside plant cells are unknown. In the present study, we selected 61 putative effector genes that are highly induced in appressoria and/or biotrophic hyphae. We then used Agrobacterium-mediated transformation to transiently express them as N-terminal fusions with fluorescent proteins in cells of Nicotiana benthamiana for imaging by confocal microscopy. Plant compartments labeled by the fusion proteins in N. benthamiana were validated by co-localization with specific organelle markers, by transient expression of the proteins in the true host plant, Arabidopsis thaliana, and by transmission electron microscopy-immunogold labeling. Among those proteins for which specific subcellular localizations could be verified, nine were imported into plant nuclei, three were imported into the matrix of peroxisomes, three decorated cortical microtubule arrays and one labeled Golgi stacks. Two peroxisome-targeted proteins harbored canonical C-terminal tripeptide signals for peroxisome import via the PTS1 (peroxisomal targeting signal 1) pathway, and we showed that these signals are essential for their peroxisome localization. Our findings provide valuable information about which host processes are potentially manipulated by this pathogen, and also reveal plant peroxisomes, microtubules, and Golgi as novel targets for fungal effectors.
DOI: 10.3389/fpls.2018.00562
PubMed: 29770142
PubMed Central: PMC5942036
Affiliations:
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Robin</name><affiliation wicri:level="3"><nlm:affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Kleemann, Jochen" sort="Kleemann, Jochen" uniqKey="Kleemann J" first="Jochen" last="Kleemann">Jochen Kleemann</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Neumann, Ulla" sort="Neumann, Ulla" uniqKey="Neumann U" first="Ulla" last="Neumann">Ulla Neumann</name><affiliation wicri:level="1"><nlm:affiliation>Central Microscopy, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Central Microscopy, Max Planck Institute for Plant Breeding Research, Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Cabre, Lisa" sort="Cabre, Lisa" uniqKey="Cabre L" first="Lisa" last="Cabre">Lisa Cabre</name><affiliation wicri:level="3"><nlm:affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Dallery, Jean Felix" sort="Dallery, Jean Felix" uniqKey="Dallery J" first="Jean-Félix" last="Dallery">Jean-Félix Dallery</name><affiliation wicri:level="3"><nlm:affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Lapalu, Nicolas" sort="Lapalu, Nicolas" uniqKey="Lapalu N" first="Nicolas" last="Lapalu">Nicolas Lapalu</name><affiliation wicri:level="3"><nlm:affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="O Connell, Richard J" sort="O Connell, Richard J" uniqKey="O Connell R" first="Richard J" last="O'Connell">Richard J. O'Connell</name><affiliation wicri:level="3"><nlm:affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The genome of the hemibiotrophic anthracnose fungus, <i>Colletotrichum higginsianum</i>, encodes a large inventory of putative secreted effector proteins that are sequentially expressed at different stages of plant infection, namely appressorium-mediated penetration, biotrophy and necrotrophy. However, the destinations to which these proteins are addressed inside plant cells are unknown. In the present study, we selected 61 putative effector genes that are highly induced in appressoria and/or biotrophic hyphae. We then used <i>Agrobacterium</i>-mediated transformation to transiently express them as <i>N</i>-terminal fusions with fluorescent proteins in cells of <i>Nicotiana benthamiana</i> for imaging by confocal microscopy. Plant compartments labeled by the fusion proteins in <i>N. benthamiana</i> were validated by co-localization with specific organelle markers, by transient expression of the proteins in the true host plant, <i>Arabidopsis thaliana</i>, and by transmission electron microscopy-immunogold labeling. Among those proteins for which specific subcellular localizations could be verified, nine were imported into plant nuclei, three were imported into the matrix of peroxisomes, three decorated cortical microtubule arrays and one labeled Golgi stacks. Two peroxisome-targeted proteins harbored canonical <i>C</i>-terminal tripeptide signals for peroxisome import <i>via</i> the PTS1 (peroxisomal targeting signal 1) pathway, and we showed that these signals are essential for their peroxisome localization. Our findings provide valuable information about which host processes are potentially manipulated by this pathogen, and also reveal plant peroxisomes, microtubules, and Golgi as novel targets for fungal effectors.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29770142</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Subcellular Localization Screening of <i>Colletotrichum higginsianum</i> Effector Candidates Identifies Fungal Proteins Targeted to Plant Peroxisomes, Golgi Bodies, and Microtubules.</ArticleTitle><Pagination><MedlinePgn>562</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2018.00562</ELocationID><Abstract><AbstractText>The genome of the hemibiotrophic anthracnose fungus, <i>Colletotrichum higginsianum</i>, encodes a large inventory of putative secreted effector proteins that are sequentially expressed at different stages of plant infection, namely appressorium-mediated penetration, biotrophy and necrotrophy. However, the destinations to which these proteins are addressed inside plant cells are unknown. In the present study, we selected 61 putative effector genes that are highly induced in appressoria and/or biotrophic hyphae. We then used <i>Agrobacterium</i>-mediated transformation to transiently express them as <i>N</i>-terminal fusions with fluorescent proteins in cells of <i>Nicotiana benthamiana</i> for imaging by confocal microscopy. Plant compartments labeled by the fusion proteins in <i>N. benthamiana</i> were validated by co-localization with specific organelle markers, by transient expression of the proteins in the true host plant, <i>Arabidopsis thaliana</i>, and by transmission electron microscopy-immunogold labeling. Among those proteins for which specific subcellular localizations could be verified, nine were imported into plant nuclei, three were imported into the matrix of peroxisomes, three decorated cortical microtubule arrays and one labeled Golgi stacks. Two peroxisome-targeted proteins harbored canonical <i>C</i>-terminal tripeptide signals for peroxisome import <i>via</i> the PTS1 (peroxisomal targeting signal 1) pathway, and we showed that these signals are essential for their peroxisome localization. Our findings provide valuable information about which host processes are potentially manipulated by this pathogen, and also reveal plant peroxisomes, microtubules, and Golgi as novel targets for fungal effectors.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Robin</LastName><ForeName>Guillaume P</ForeName><Initials>GP</Initials><AffiliationInfo><Affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kleemann</LastName><ForeName>Jochen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Neumann</LastName><ForeName>Ulla</ForeName><Initials>U</Initials><AffiliationInfo><Affiliation>Central Microscopy, Max Planck Institute for Plant Breeding Research, Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cabre</LastName><ForeName>Lisa</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dallery</LastName><ForeName>Jean-Félix</ForeName><Initials>JF</Initials><AffiliationInfo><Affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lapalu</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>O'Connell</LastName><ForeName>Richard J</ForeName><Initials>RJ</Initials><AffiliationInfo><Affiliation>UMR BIOGER, Institut National de la Recherche Agronomique, AgroParisTech, Université Paris-Saclay, Versailles, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>05</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Colletotrichum</Keyword><Keyword MajorTopicYN="N">Golgi</Keyword><Keyword MajorTopicYN="N">effectoromics</Keyword><Keyword MajorTopicYN="N">fungus</Keyword><Keyword MajorTopicYN="N">localization</Keyword><Keyword 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Robin</name></region><name sortKey="Cabre, Lisa" sort="Cabre, Lisa" uniqKey="Cabre L" first="Lisa" last="Cabre">Lisa Cabre</name><name sortKey="Dallery, Jean Felix" sort="Dallery, Jean Felix" uniqKey="Dallery J" first="Jean-Félix" last="Dallery">Jean-Félix Dallery</name><name sortKey="Lapalu, Nicolas" sort="Lapalu, Nicolas" uniqKey="Lapalu N" first="Nicolas" last="Lapalu">Nicolas Lapalu</name><name sortKey="O Connell, Richard J" sort="O Connell, Richard J" uniqKey="O Connell R" first="Richard J" last="O'Connell">Richard J. O'Connell</name></country><country name="Allemagne"><noRegion><name sortKey="Kleemann, Jochen" sort="Kleemann, Jochen" uniqKey="Kleemann J" first="Jochen" last="Kleemann">Jochen Kleemann</name></noRegion><name sortKey="Neumann, Ulla" sort="Neumann, Ulla" uniqKey="Neumann U" first="Ulla" last="Neumann">Ulla Neumann</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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